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In HEK293 cells expressing WT, predominant corin staining was on the cell surface area . Such staining was absent in vector-transfected cells. In cells expressing the insA variant, corin staining was more prominent intracellularly. By co-staining with TGN46, a Golgi marker, a significant portion of the insA variant was found in the Golgi . In parallel experiments of co-staining with PDI, an ER marker, no significant staining of WT or the variant was within the ER . The info reveal that the insA variant was much less efficient in trafficking through the Golgi network.Figure 5: Subcellular distribution of corin proteins. HEK293 cells expressing WT and the insA variant were stained for corin and TGN46, a Golgi marker .